Exposure of human peripheral blood lymphocytes to electromagnetic fields associated with cellular phones leads to chromosomal instability

Maya Mashevich 1 3, Dan Folkman 2, Amit Kesar 2, Alexander Barbul 3, Rafi Korenstein 3 *, Eli Jerby 2, Lydia Avivi 1

Keywords

continuous RF fields • nonthermal effects • aneuploidy • centromeric DNA replication • carcinogenesis

Abstract

Whether exposure to radiation emitted from cellular phones poses a health hazard is at the focus of current debate. We have examined whether in vitro exposure of human peripheral blood lymphocytes (PBL) to continuous 830 MHz electromagnetic fields causes losses and gains of chromosomes (aneuploidy), a major somatic mutation leading to genomic instability and thereby to cancer. PBL were irradiated at different average absorption rates (SAR) in the range of 1.6-8.8 W/kg for 72 hr in an exposure system based on a parallel plate resonator at temperatures ranging from 34.5-37.5 °C. The averaged SAR and its distribution in the exposed tissue culture flask were determined by combining measurements and numerical analysis based on a finite element simulation code. A linear increase in chromosome 17 aneuploidy was observed as a function of the SAR value, demonstrating that this radiation has a genotoxic effect. The SAR dependent aneuploidy was accompanied by an abnormal mode of replication of the chromosome 17 region engaged in segregation (repetitive DNA arrays associated with the centromere), suggesting that epigenetic alterations are involved in the SAR dependent genetic toxicity. Control experiments (i.e., without any RF radiation) carried out in the temperature range of 34.5-38.5 °C showed that elevated temperature is not associated with either the genetic or epigenetic alterations observed following RF radiation – the increased levels of aneuploidy and the modification in replication of the centromeric DNA arrays. These findings indicate that the genotoxic effect of the electromagnetic radiation is elicited via a non-thermal pathway. Moreover, the fact that aneuploidy is a phenomenon known to increase the risk for cancer, should be taken into consideration in future evaluation of exposure guidelines. Bioelectromagnetics 24:82-90, 2003. © 2003 Wiley-Liss, Inc.

Linus Pauling emphasized in our discussions years ago that Vitamin C excreted in the urine did not mean you had too much Vitamin C. He argued that maintaining high plasma vitamin C had all sorts of positive effects. Reduction in the risk of stroke is one of them. A couple of glasses of fresh orange juice a day contains enough vitamin C to cut stroke risk in half. Pauling felt that significantly more vitamin C in the bloodstream had far reaching systemic effects.

Plasma Vitamin C Modifies the Association Between Hypertension and Risk of Stroke

S. Kurl, MD; T.P. Tuomainen, MD; J.A. Laukkanen, MD; K. Nyyssönen, PhD; T. Lakka, MD, PhD; J. Sivenius, MD, PhD; J.T. Salonen, MD, PhD, MscPH

Stroke. 2002;33:1568.

Background and Purpose— There are no prospective studies to determine whether plasma vitamin C modifies the risk of stroke among hypertensive and overweight individuals. We sought to examine whether plasma vitamin C modifies the association between overweight and hypertension and the risk of stroke in middle-aged men from eastern Finland.

Methods— We conducted a 10.4-year prospective population-based cohort study of 2419 randomly selected middle-aged men (42 to 60 years) with no history of stroke at baseline examination. A total of 120 men developed a stroke, of which 96 were ischemic and 24 hemorrhagic strokes.

Results— Men with the lowest levels of plasma vitamin C (<28.4 µmol/L, lowest quarter) had a 2.4-fold (95% CI, 1.4 to 4.3; P=0.002) risk of any stroke compared with men with highest levels of plasma vitamin C (>64.96 µmol/L, highest quarter) after adjustment for age and examination months. An additional adjustment for body mass index, systolic blood pressure, smoking, alcohol consumption, serum total cholesterol, diabetes, and exercise-induced myocardial ischemia attenuated the association marginally (relative risk, 2.1; 95% CI, 1.2 to 3.8; P=0.01). Adjustment for prevalent coronary heart disease and atrial fibrillation did not attenuate the association any further. Furthermore, hypertensive men with the lowest vitamin C levels (<28.4 µmol/L) had a 2.6-fold risk (95% CI, 1.52 to 4.48; P<0.001), and overweight men (25 kg/m2) with low plasma vitamin C had a 2.7-fold risk (95% CI, 1.48 to 4.90; P=0.001) for any stroke after adjustment for age, examination months, and other risk factors. Conclusions— Low plasma vitamin C was associated with increased risk of stroke, especially among hypertensive and overweight men.

Grandma used to give the kids cod liver oil because it helped out the immune system. It tasted bad but it worked. These days, there are a lot of toxins in fish and concentrating the oil must be done by molecular distillation to avoid concentrating the toxins along with the oil.

Before I comment further on fish oil, it is important to note that the best supplement for immune function is Transfer Factor Plus. Research shows that nothing comes close to its effectiveness and I can vouch for it personally as I take it every day. It is the only supplement I have set up a web page for because it is critical that anyone with any chronic disease be supplementing their immune system. You can order it by clicking here, and it is as safe for kids as for adults. For the little ones it will prevent or help recover from colds and flu. For adults, it could save your life.

The next most important supplement to take is pharmaceutical grade fish oil. The best brand is produced by Dr. Sears Labs and is expensive. I take it because it has significant effects on multiple facets of body function. It is particularly good at lowering the heart rate (great for runners) and reducing the risk of heart disease in general. There is an inexpensive Canadian brand of pharmaceutical grade oil sold at www.iherb.com, Rx Omega 3 Factors. Several people have sent me email asking where they can find it, so I am posting the link here.

I have thousands of supplements that I have tested in my laboratory at home and I only take four things regularly: Transfer Factor, pharmaceutical grade fish oil, a really good vitamin supplement, and Horizon Low Fat Cottage Cheese mixed with Barlean’s flax seed oil for cancer prevention (the only combination that tastes good). Vitamin supplements need to be commented on separately, as these merit a longer discussion.

Fish consumption and risk of stroke in men.

He K, Rimm EB, Merchant A, Rosner BA, Stampfer MJ, Willett WC, Ascherio A.

JAMA 2002 Dec 25;288(24):3130-6

Department of Nutrition, Harvard School of Public Health, 665 Huntington Ave, Boston, Mass 02115, USA. [email protected]

CONTEXT: The effect of fish consumption or long-chain omega-3 polyunsaturated fatty acid (PUFA) intake on risk of stroke remains uncertain.

OBJECTIVE: To examine the relation of fish consumption and long-chain omega-3 PUFA intake and risk of stroke in men.

DESIGN AND SETTING: The Health Professional Follow-up Study, a US prospective cohort study with 12 years of follow-up.

PARTICIPANTS: A total of 43671 men aged 40 to 75 years who completed a detailed and validated semiquantitative food frequency questionnaire and who were free of cardiovascular disease at baseline in 1986.

MAIN OUTCOME MEASURE: Relative risk (RR) of stroke by subtype based on cumulative average fish consumption or long-chain omega-3 PUFA intake, ascertained in 1986, 1990, and 1994.

RESULTS: We documented 608 strokes during the 12-year follow-up period, including 377 ischemic, 106 hemorrhagic, and 125 unclassified strokes. Compared with men who consumed fish less than once per month, the multivariate RR of ischemic stroke was significantly lower among those who ate fish 1 to 3 times per month (RR, 0.57; 95% confidence interval [CI], 0.35-0.95). However, a higher frequency of fish intake was not associated with further risk reduction; the RR was 0.54 (95% CI, 0.31-0.94) for men who consumed fish 5 or more times per week. This lack of linearity was confirmed by spline analyses. By dichotomized fish intake, the multivariate RR for men who consumed fish at least once per month compared with those who ate fish less than once per month was 0.56 (95% CI, 0.38-0.83) for ischemic stroke and 1.36 (95% CI, 0.48-3.82) for hemorrhagic stroke. The inverse association between fish intake and risk of ischemic stroke was not materially modified by use of aspirin. No significant associations were found between fish or long-chain omega-3 PUFA intake and risk of hemorrhagic stroke.

CONCLUSION: Our findings suggest that eating fish once per month or more can reduce the risk of ischemic stroke in men.

Bill Moyers was one of the study subjects in a recent study of body burden of industrial pollutants. His blood had over 30 different types of PCBs and a host of other contaminants.

BodyBurden: The Pollution in People

In a study led by Mount Sinai School of Medicine in New York, in collaboration with the Environmental Working Group and Commonweal, researchers at two major laboratories found an average of 91 industrial compounds, pollutants, and other chemicals in the blood and urine of nine volunteers, with a total of 167 chemicals found in the group. Like most of us, the people tested do not work with chemicals on the job and do not live near an industrial facility.

Scientists refer to this contamination as a person’s body burden. Of the 167 chemicals found, 76 cause cancer in humans or animals, 94 are toxic to the brain and nervous system, and 79 cause birth defects or abnormal development. The dangers of exposure to these chemicals in combination has never been studied.

Superbug killer found in rockpool
CNN, Thursday, February 27, 2003 Posted: 5:35 AM EST (1035 GMT)
EDINBURGH, Scotland — Scientists may have found the answer to Britain’s most dangerous hospital superbug — in slime taken from Scottish rock pools.

A reader sent me the superbug CNN article when it was published last Thursday. Medical error is the third leading cause of death in the United State and a major component of that error is nosocomial infections (you get sick because you are in the hospital with bugs you did not have when you entered the hospital). Dr. Starfield, at Johns Hopkins, reports that there are about 80,000 deaths each year from nosocomial infections in the U.S. alone. CNN notes that the staphylococcus bacteria accounts for almost half of all UK hospital infections. In the U.S., MRSA accounts for about 25% of infections, or 20,000 deaths a year. The drug companies are hot on the trail of yet a better antibiotic.

There are so many interesting questions about this bug and avoidance or treatment of antibiotic-resistant infections in general that I will take several postings to comment on even a few items.

1. What is the electronic resonant frequency of MRSA, and can it easily be killed by a frequency generator or Rife machine?
2. Why are nasty bugs like this most prevalent in hospitals?
3. What alternative strategies could we take to avoid evolving nasty, resistant bug strains in the future?
4. What easy nutritional aid could be given to hospital patients that is proven in clinical trials and would drastically reduce the number of infections by this bug. What is the probability of getting this nutritional aid if you go into the hospital? Is failure to provide proven treatments common in medicine today? Is failure to provide this assistance malpractice?
5. Do you have this bug in your system? Should you eliminate it? What could you do if you get it and treatment with antibiotics is futile?

It is well known in the homeopathic lore that one disease tends to come to the foreground and others are suppressed. My experiments indicate that many of the symptoms used for homeopathic diagnosis are related to micoorganisms and that giving the right remedy kills or moves around the microorganisms changing the symptoms. In any event, I spent 8 years funded by the National Cancer Institute doing research on colon cancer and here is an example where one disease suppresses another.

The epidemilogy of colon cancer showed that it was less in underdeveloped countries and one of the theories was that a high fiber diet was the cause. Well fiber may be good for you as well, but a new study shows that a bad bug, EPEC, induces resistance to colon cancer. EPEC is a significant health threat in the developing world and causes sporadic but deadly outbreaks of hemorrhagic colitis and hemolytic-uremic syndrome in North America and other developed areas.

Coliforms crush colon cancer: Bacterial enterotoxins can induce resistance to colorectal cancer.

By Tudor P Toma, The Scientist Daily News, 11 Feb 2003

The incidence of colorectal cancer — a major cause of mortality in industrialized nations — is lowest in developing countries, where high levels of enterotoxigenic Escherichia coli (ETEC) infection are seen. Whilst it has been thought that there is a link between cancer and ETEC, the mechanisms that underlie this inverse relationship have been poorly understood. In the February 10 Proceedings of the National Academy of Sciences, G. M. Pitari and colleagues at the Thomas Jefferson University, Philadelphia, USA, show that a bacterial heat-stable enterotoxin suppresses colon cancer cell proliferation by a guanylyl cyclase C-mediated (GC-C) signaling cascade.

In discussions with FSCAN owners, some users are concerned if they test positive for a known organism. Many organisms are quite common and you will probably find them if you scan for their frequencies. Heliocobacter pylori is a good example. Most people have this organism is their bodies in small quantities and do not have ulcers, even though this organism causes ulcers.

However, if you scan and find this organism and then eliminate it, you remove virtually all of the risk of getting an ulcer. If every one did this, it could eliminate more than 50% of the ulcers in the population, maybe close to 100%.

How bacteria induce stomach ulcers: Bacterial cytotoxin induces the epithelial cell detachment that leads to ulcer formation. By Jonathan Weitzman

Helicobacter pylori is a Gram-positive bacterium that colonizes the gut mucosa and can induce gastric ulcers. In an Advanced Online Publication in Nature Genetics, Akihiro Fujikawa and colleagues report how the H. pylori cytotoxin VacA causes ulcer formation (Nature Genetics, DOI:10.1038/ng1112, 24 February, 2003).

It is ironic that the recently DNA sequenced “cancer germ” is exempted from toxic control by EPA because it is so ubiquitous in the environment!

Arrow scientific reports that:

Bacillus licheniformis is a Gram-positive, motile, spore-forming, facultatively anaerobic rod. Information regarding Bacillus licheniformis in relation to food safety is limited. Food poisoning by Bacillus licheniformis is characterised by diarrhoea, although vomiting occurs in half of reported cases. Bacillus licheniformis food poisoning has been associated with cooked meat, poultry and vegetable dishes (particularly stews and curries which have been served with rice).

I have seen everyone eating a good steak dinner become infected with this organism. You must take responsibility for your own health as your doctor will not test for this infection and the government does not control the use of this organism in industry. In the 1940’s, New York hospitals were curing ulcers with antibiotics. It took over 50 years before the medical community would consider that an ulcer could be caused by a microorganism. I suspect that it will be at least 2050 before this organism is accepted as a causative factor in most cancers. Meanwhile, you are on your own.

Bacillus Licheniformis TSCA Section 5(h)(4) Exemption: Final Decision Document

1. Risks from use of the recipient microorganism B. licheniformis are low. B. licheniformis is ubiquitous in the environment and the releases expected from fermentation facilities will not significantly increase populations of this microorganism in the environment. Although the possibility of human infection by B. licheniformis is not non-existent, it is low in the industrial setting, because it occurs primarily in highly immunocompromised individuals. Infection might be a possibility following trauma, but in the industrial setting with the use of proper safety precautions, good laboratory practices, and proper protective clothing and eyewear, the potential for infection of workers should be quite low. Although B. licheniformis may be associated with livestock abortions, the use of this microorganism in fermentation facilities will not substantially increase the frequency of this occurrence.

2. Use of strains of B. licheniformis which are eligible for the TSCA section 5(h)(4) exemption present no unreasonable risk. While not completely innocuous, B. licheniformis presents low risk of adverse effects to human health or the environment. Because the recipient microorganism was found to have little potential for adverse effects, introduced genetic material meeting the specified criteria would not likely significantly increase potential for adverse effects. As further assurance that risks would be low, EPA is specifying procedures for minimizing numbers of organisms emitted from the facility for the Tier I exemption and will be reviewing the conditions selected for the Tier II exemption.

Bacillus licheniformis in fungus-like form. Photo by Milton Wainwright

A tremendously significant finding was published in Lancet this month. What appears to be the Rife “filterable bacteria” was isolated and DNA sequenced by British researchers. Bacillus licheniformis is a pleomorphic organism that appears as rods, cocci, and fungus-like forms. Rife had a very difficult time culturing this organism in the 1920’s and people have had limited success since then, so demonstrating non-contaminated multiple forms of the same organism with exactly the same DNA sequence is a major accomplish that could end decades of controversy.

Sansom, Clare. “Cancer Germ” Bacteria Isolated. THE LANCET Oncology, Vol 4 February 2003, p. 63.

(You will need to create a free Lancet account to view this document.)

Milton Wainwright had already published data previously showing bacteria can pass through very small holes (as noted by Rife) and that this has major implications for their role as pathogens. See: Med Hypotheses 2002 Jun;58(6):558-60.

In my view, scanning and eliminating this bacterium in the general population could reduce the incidence of cancer and improve survival of cancer patients by more than 50%. This is based on my on my own research and that of hundreds of investigators trying to replicate Rife’s early work on successful treatment of cancer patients with electronic devices.

The reason this finding is extremely important is that the incidence of cancer has been increasing for the past few decades and no significant reduction in overall survival rates has been achieved since Richard Nixon launched the multi-billion dollar “war on cancer” a generation ago. My Ph.D. thesis advisor and co-author (a MacArthur Fellow and former Editor of the Journal of the National Cancer Institute, and statistical editor of the New England Journal of Medicine) reported no signficant increase in survival rates in cancer patients some years ago, and I don’t think things have changed much since, except for isolated cancer types.

Bailar, JC 3rd, Smith EM. Have we reduced the risk of getting cancer or of dying from cancer? An update. Med Oncol Tumor Pharmacother 1987;4(3-4):193-8.

We have examined trends in cancer mortality, incidence and survival in the United States to update our earlier work and respond to criticisms. [Bailar, J.C., Smith, E.M.: New Engl. J. Med. 314, 1226 (1986).] Here we concentrate on the years 1975-1984, and show that overall cancer mortality has increased, incidence has increased and case survival is virtually unchanged. This generally unfavorable picture is scarcely changed when lung cancer is excluded from the trends. While trends for individual cancers have been mixed, overall progress in both curative treatment and prevention has been minimal. This evaluation does not deny the marked progress in treating some uncommon forms of cancer, improved palliation, reduced extent or severity of treatment, or benefits of cancer research that can be applied in other areas of medicine. While our finding of limited progress is not new, we believe that it requires increased attention in setting the course of future research initiatives, demonstration programs, medical training and clinical practice.

A key area of future research is developing the means to eliminate the Rife pathogen, now almost certainly known to be Bacillus licheniformis, and electronic medicine is the most effective means at present.

There is a circular magnetic disk attachment to the FSCAN that is useful for eliminating pathogens. Previous studies mentioned on this site have noted that both magnetic and electrical oscillating fields affect microorganisms with the magnetic field being the most important, at least for some organisms. Here is a study from Washington University showing how this effect can be used to treat malaria.

The approach in the article does not eliminate all the malaria parasite, but enough to control it. There is a good argument as to the mechanism. To eliminate all of the malaria parasite, you would need the exact frequency of the parasite for each stage of its life cycle and use plate zapping to eliminate it in places where it would hide out.

Magnets may revolutionize malaria treatment

Researchers at the University of Washington have discovered a method of treating malaria with magnetic fields that could prove revolutionary in controlling the disease the World Health Organization calls one of the world’s most complex and serious human health concerns.

Henry Lai, UW research professor of bioengineering, says the malaria parasite Plasmodium appears to lose vigor and can die when exposed to oscillating magnetic fields, which Lai thinks may cause tiny iron-containing particles inside the parasite to move in ways that damage the organism.

“If further studies confirm our findings and their application in animals and people, this would be an inexpensive and simple way to treat a disease that affects 500 million people every year, almost all in third-world countries,” Lai said. According to the World Health Organization, as many as 2.7 million people die of malaria every year, approximately 1 million of those children.